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expression plasmid pmscv puro ires gfp 64  (Addgene inc)


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    Structured Review

    Addgene inc expression plasmid pmscv puro ires gfp 64
    Expression Plasmid Pmscv Puro Ires Gfp 64, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmscv-pig/pMSCV+PIG+(Puro+IRES+GFP+empty+vector)+(Plasmid+%2321654)/10__1016_slash_j__isci__2026__115182-641-41-45
    Average 93 stars, based on 77 article reviews
    expression plasmid pmscv puro ires gfp 64 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Cytoplasmic linker protein CLIP170 negatively regulates the TLR4 signaling by targeting the TLR adaptor protein TIRAP
    Article Snippet: To overexpress CLIP170 in mouse macrophages, full-length CLIP170 cds segment was ligated to the lentivirus expression plasmid, pMSCV-PIG (a gift from David Bartel; Addgene plasmid # 21654.

    Article Title: Cytoplasmic linker protein CLIP170 negatively regulates the TLR4 signaling by targeting the TLR adaptor protein TIRAP
    Article Snippet: Over expression and silencing of CLIP170 in mouse macrophages To overexpress CLIP170 in mouse macrophages, full-length CLIP170 cds segment was ligated to the lentivirus expression plasmid, pMSCV-PIG (a gift from David Bartel; Addgene plasmid # 21654.

    Article Title: Rapid Generation of MicroRNA Sponges for MicroRNA Inhibition
    Article Snippet: The pMSCV-PIG-sp vector was generated by inserting a SanD1 restriction site containing linker ( ) between the XhoI and EcoRI restriction sites of pMSCV-PIG (Addgene, www.addgene.org , plasmid 21654). pMSCV-PIG-sp was digested with SanD1 for 1 h at 37°C (Fermentas, St. Leon-Rot, Germany) and dephosphorylated with 1 μl CIAP (1 U/ul, Invitrogen, Carlsbad, CA, USA) for 5 minutes at 37°C.

    Generated:

    Article Title: Cytoplasmic linker protein CLIP170 negatively regulates the TLR4 signaling by targeting the TLR adaptor protein TIRAP
    Article Snippet: To overexpress CLIP170 in mouse macrophages, full-length CLIP170 cds segment was ligated to the lentivirus expression plasmid, pMSCV-PIG (a gift from David Bartel; Addgene plasmid # 21654.

    Article Title: Cytoplasmic linker protein CLIP170 negatively regulates the TLR4 signaling by targeting the TLR adaptor protein TIRAP
    Article Snippet: Over expression and silencing of CLIP170 in mouse macrophages To overexpress CLIP170 in mouse macrophages, full-length CLIP170 cds segment was ligated to the lentivirus expression plasmid, pMSCV-PIG (a gift from David Bartel; Addgene plasmid # 21654.

    Article Title: Rapid Generation of MicroRNA Sponges for MicroRNA Inhibition
    Article Snippet: The pMSCV-PIG-sp vector was generated by inserting a SanD1 restriction site containing linker ( ) between the XhoI and EcoRI restriction sites of pMSCV-PIG (Addgene, www.addgene.org , plasmid 21654). pMSCV-PIG-sp was digested with SanD1 for 1 h at 37°C (Fermentas, St. Leon-Rot, Germany) and dephosphorylated with 1 μl CIAP (1 U/ul, Invitrogen, Carlsbad, CA, USA) for 5 minutes at 37°C.

    Binding Assay:

    Article Title: Cytoplasmic linker protein CLIP170 negatively regulates the TLR4 signaling by targeting the TLR adaptor protein TIRAP
    Article Snippet: To overexpress CLIP170 in mouse macrophages, full-length CLIP170 cds segment was ligated to the lentivirus expression plasmid, pMSCV-PIG (a gift from David Bartel; Addgene plasmid # 21654.

    Article Title: Cytoplasmic linker protein CLIP170 negatively regulates the TLR4 signaling by targeting the TLR adaptor protein TIRAP
    Article Snippet: Over expression and silencing of CLIP170 in mouse macrophages To overexpress CLIP170 in mouse macrophages, full-length CLIP170 cds segment was ligated to the lentivirus expression plasmid, pMSCV-PIG (a gift from David Bartel; Addgene plasmid # 21654.

    Article Title: Rapid Generation of MicroRNA Sponges for MicroRNA Inhibition
    Article Snippet: The pMSCV-PIG-sp vector was generated by inserting a SanD1 restriction site containing linker ( ) between the XhoI and EcoRI restriction sites of pMSCV-PIG (Addgene, www.addgene.org , plasmid 21654). pMSCV-PIG-sp was digested with SanD1 for 1 h at 37°C (Fermentas, St. Leon-Rot, Germany) and dephosphorylated with 1 μl CIAP (1 U/ul, Invitrogen, Carlsbad, CA, USA) for 5 minutes at 37°C.

    Ligation:

    Article Title: Cytoplasmic linker protein CLIP170 negatively regulates the TLR4 signaling by targeting the TLR adaptor protein TIRAP
    Article Snippet: To overexpress CLIP170 in mouse macrophages, full-length CLIP170 cds segment was ligated to the lentivirus expression plasmid, pMSCV-PIG (a gift from David Bartel; Addgene plasmid # 21654.

    Article Title: Cytoplasmic linker protein CLIP170 negatively regulates the TLR4 signaling by targeting the TLR adaptor protein TIRAP
    Article Snippet: Over expression and silencing of CLIP170 in mouse macrophages To overexpress CLIP170 in mouse macrophages, full-length CLIP170 cds segment was ligated to the lentivirus expression plasmid, pMSCV-PIG (a gift from David Bartel; Addgene plasmid # 21654.

    Article Title: Rapid Generation of MicroRNA Sponges for MicroRNA Inhibition
    Article Snippet: The pMSCV-PIG-sp vector was generated by inserting a SanD1 restriction site containing linker ( ) between the XhoI and EcoRI restriction sites of pMSCV-PIG (Addgene, www.addgene.org , plasmid 21654). pMSCV-PIG-sp was digested with SanD1 for 1 h at 37°C (Fermentas, St. Leon-Rot, Germany) and dephosphorylated with 1 μl CIAP (1 U/ul, Invitrogen, Carlsbad, CA, USA) for 5 minutes at 37°C.

    Clone Assay:

    Article Title: Cytoplasmic linker protein CLIP170 negatively regulates the TLR4 signaling by targeting the TLR adaptor protein TIRAP
    Article Snippet: To overexpress CLIP170 in mouse macrophages, full-length CLIP170 cds segment was ligated to the lentivirus expression plasmid, pMSCV-PIG (a gift from David Bartel; Addgene plasmid # 21654.

    Article Title: Cytoplasmic linker protein CLIP170 negatively regulates the TLR4 signaling by targeting the TLR adaptor protein TIRAP
    Article Snippet: Over expression and silencing of CLIP170 in mouse macrophages To overexpress CLIP170 in mouse macrophages, full-length CLIP170 cds segment was ligated to the lentivirus expression plasmid, pMSCV-PIG (a gift from David Bartel; Addgene plasmid # 21654.

    Article Title: Rapid Generation of MicroRNA Sponges for MicroRNA Inhibition
    Article Snippet: The pMSCV-PIG-sp vector was generated by inserting a SanD1 restriction site containing linker ( ) between the XhoI and EcoRI restriction sites of pMSCV-PIG (Addgene, www.addgene.org , plasmid 21654). pMSCV-PIG-sp was digested with SanD1 for 1 h at 37°C (Fermentas, St. Leon-Rot, Germany) and dephosphorylated with 1 μl CIAP (1 U/ul, Invitrogen, Carlsbad, CA, USA) for 5 minutes at 37°C.

    Over Expression:

    Article Title: Cytoplasmic linker protein CLIP170 negatively regulates the TLR4 signaling by targeting the TLR adaptor protein TIRAP
    Article Snippet: To overexpress CLIP170 in mouse macrophages, full-length CLIP170 cds segment was ligated to the lentivirus expression plasmid, pMSCV-PIG (a gift from David Bartel; Addgene plasmid # 21654.

    Article Title: Cytoplasmic linker protein CLIP170 negatively regulates the TLR4 signaling by targeting the TLR adaptor protein TIRAP
    Article Snippet: Over expression and silencing of CLIP170 in mouse macrophages To overexpress CLIP170 in mouse macrophages, full-length CLIP170 cds segment was ligated to the lentivirus expression plasmid, pMSCV-PIG (a gift from David Bartel; Addgene plasmid # 21654.

    Article Title: Rapid Generation of MicroRNA Sponges for MicroRNA Inhibition
    Article Snippet: The pMSCV-PIG-sp vector was generated by inserting a SanD1 restriction site containing linker ( ) between the XhoI and EcoRI restriction sites of pMSCV-PIG (Addgene, www.addgene.org , plasmid 21654). pMSCV-PIG-sp was digested with SanD1 for 1 h at 37°C (Fermentas, St. Leon-Rot, Germany) and dephosphorylated with 1 μl CIAP (1 U/ul, Invitrogen, Carlsbad, CA, USA) for 5 minutes at 37°C.

    Expressing:

    Article Title: Cytoplasmic linker protein CLIP170 negatively regulates the TLR4 signaling by targeting the TLR adaptor protein TIRAP
    Article Snippet: To overexpress CLIP170 in mouse macrophages, full-length CLIP170 cds segment was ligated to the lentivirus expression plasmid, pMSCV-PIG (a gift from David Bartel; Addgene plasmid # 21654.

    Article Title: Cytoplasmic linker protein CLIP170 negatively regulates the TLR4 signaling by targeting the TLR adaptor protein TIRAP
    Article Snippet: Over expression and silencing of CLIP170 in mouse macrophages To overexpress CLIP170 in mouse macrophages, full-length CLIP170 cds segment was ligated to the lentivirus expression plasmid, pMSCV-PIG (a gift from David Bartel; Addgene plasmid # 21654.

    Article Title: Rapid Generation of MicroRNA Sponges for MicroRNA Inhibition
    Article Snippet: The pMSCV-PIG-sp vector was generated by inserting a SanD1 restriction site containing linker ( ) between the XhoI and EcoRI restriction sites of pMSCV-PIG (Addgene, www.addgene.org , plasmid 21654). pMSCV-PIG-sp was digested with SanD1 for 1 h at 37°C (Fermentas, St. Leon-Rot, Germany) and dephosphorylated with 1 μl CIAP (1 U/ul, Invitrogen, Carlsbad, CA, USA) for 5 minutes at 37°C.



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